Journal:

Article Title: Identification of Mycobacterium Species by PCR-Restriction Fragment Length Polymorphism Analyses Using Fluorescence Capillary Electrophoresis

doi:

Figure Lengend Snippet: Mycobacterium species tested

Article Snippet: Nonmycobacterial species included Corynebacterium diphtheriae , Tsukamurella sp., Corynebacterium pseudotuberculosis , Nocardia brasiliensis , and Gordona sputi ( 3 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species No. of isolates tested Reference strains a M. tuberculosis 16 TMC 119 (ATCC 35810) M. bovis 10 TMC 412 (ATCC 35726) M. bovis BCG (BCG Tice) 10 TMC 1028 (ATCC 35743) M. africanum 2 CDC72-1432 M. microti 2 TMC 1608 (ATCC 35782) M. avium 10 TMC 716 (ATCC 35717) M. intracellulare 3 TMC 1469 (ATCC 35772) M. simiae 7 TMC 1226 (ATCC 25275) M. gordonae 13 TMC 1324 (ATCC 14470) M. kansasii 10 TMC 1214 (ATCC 35777) M. fortuitum 5 CDC85-1098 M. peregrinum 3 TMC 1547 (ATCC 14467) M. chelonae 5 TMC 1524 (ATCC 35749) M. abscessus 5 TMC 1542 (ATCC 35751) M. celatum 9 ATCC 51131 M. marinum 10 909 b M. asiaticum 8 CDC88-334 M. mucogenicum 10 CDC86-650 M. malmoense 9 01355 b M. gastri 8 ATCC 25127 M. scrofulaceum 8 CDC89-447 M. szulgai 9 954 b M. xenopi 8 CDC90-TI-723 Total 180 Open in a separate window a Strain used for development and standardization of the procedure. b Strain provided by W. R. Butler.

Techniques:

Journal: Nucleic Acids Research

Article Title: Directional transition from initiation to elongation in bacterial translation

doi: 10.1093/nar/gkv869

Figure Lengend Snippet: Effect of GTP hydrolysis on the kinetics of 70S IC maturation. 30S IC was rapidly mixed with 50S subunits (1 μM) in a stopped-flow ( A – E ) or a quench-flow ( F ) machine and the time courses of indicated reactions were monitored in the presence of GTP (black) or GTPγS (green). An inset in ( F ) shows the extended time window for peptide bond formation. Smooth lines (A–E) show fits obtained by global evaluation of all time courses using numerical integration.

Article Snippet: GTP, GDP, GTPγS, GDPNP, mant-GTP (2′/3′- O -( N -methyl-anthraniloyl)-guanosine-5′-triphosphate, triethylammonium salt) and mant-GTPγS (2′/3′- O -( N -methyl-anthraniloyl)-guanosine-5′-(γ-thio)-triphosphate, triethylammonium salt), were purchased from Jena Biosciences; Bpy-GTP (guanosine 5′-triphosphate, BODIPY FL 2′-(or-3′)- O -( N -(2-aminoethyl)urethane), trisodium salt) and Bpy-GDP (guanosine 5′-diphosphate, BODIPY FL 2′-(or-3′)- O -( N -(2-aminoethyl)urethane), bis-(triethylammonium) salt) from Life Technologies.

Techniques:

Journal: Nucleic Acids Research

Article Title: Directional transition from initiation to elongation in bacterial translation

doi: 10.1093/nar/gkv869

Figure Lengend Snippet: Effect of GTP hydrolysis on binding of IF1 and IF2 to mature 70S IC. ( A ) 30S IC formed in the presence of Bpy-Met-tRNA fMet and GTP (12.5 μM) was rapidly mixed with 50S subunits in the presence or absence of GTPγS (0.25 mM). Time courses of Bpy-Met-tRNA fMet fluorescence changes were monitored. ( B ) Interaction of Bpy-Met-tRNA fMet with IF2 upon binding of the factor to 70S IC was followed by mixing purified 70S ICs (containing Bpy-Met-tRNA fMet ) with IF2 bound to GTPγS, GDPNP, GTP or GDP. Similar experiments were performed using an IF2 variant lacking the C2-domain (ΔC2) in the presence of GTPγS. ( C ) 30S S13 (Alx488) IC formed with IF1 4 (Atto540Q) and GTP (12.5 μM) was mixed with 50S subunits in the presence or absence of GTPγS (0.25 mM). ( D ) The binding of the IF1 to mature 70S IC was followed by mixing non-purified 70S ICs (formed with 30S S13 (Alx488) in the absence of IF1) with IF1 4 (Atto540Q), in the presence of GTP or GTPγS.

Article Snippet: GTP, GDP, GTPγS, GDPNP, mant-GTP (2′/3′- O -( N -methyl-anthraniloyl)-guanosine-5′-triphosphate, triethylammonium salt) and mant-GTPγS (2′/3′- O -( N -methyl-anthraniloyl)-guanosine-5′-(γ-thio)-triphosphate, triethylammonium salt), were purchased from Jena Biosciences; Bpy-GTP (guanosine 5′-triphosphate, BODIPY FL 2′-(or-3′)- O -( N -(2-aminoethyl)urethane), trisodium salt) and Bpy-GDP (guanosine 5′-diphosphate, BODIPY FL 2′-(or-3′)- O -( N -(2-aminoethyl)urethane), bis-(triethylammonium) salt) from Life Technologies.

Techniques: Binding Assay, Fluorescence, Purification, Variant Assay